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mouse antibodies against centrin2 04-1624  (Merck KGaA)

 
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    Merck KGaA mouse antibodies against centrin2 04-1624
    Generation of CEP215-truncated mutants with defects in molecular dynamicity. (A) Schematic diagram illustrating the CEP215 protein with functional sites (CM1 and CM2), protein-binding sites, coiled-coil domains (CCDs), intrinsically disordered regions (IDRs) and the truncated mutant sites used in this study. (B) The number of cells with (w/) cytoplasmic clusters of FLAG–CEP215–CRY2-truncated mutant proteins was counted. * P <0.05 (one-way ANOVA with Tukey's post hoc test). (C) FRAP signals of the mCh–CEP215–CRY2 mutant proteins at the cytoplasm were measured for up to 600 s. w/o, without. (D) Stably expressed FLAG–mCh–CEP215 mutants in p53 KO ; CEP215 KO HeLa cells were subjected to immunoblot analyses with anti-CEP215, FLAG and GAPDH antibodies. Blot representative of four experimental repeats. (E) FRAP analyses of the FLAG–mCh–CEP215 proteins were carried out at mitotic phase centrosomes of the rescued cells. <t>GFP–centrin2</t> marks the centrosomes. The analyzed cells were photographed with differential interference contrast microscopy. Scale bars: 1 μm (FRAP); 10 μm (DIC). (F) The FRAP signals of FLAG–mCh–CEP215 (WT, ΔCCD5 and L1867S) at mitotic phase centrosomes were measured for up to 160 s. For B, C and F, at least 90 (B), 30 (C) and 10 (F) cells per experimental group were counted in three independent experiments. Values are mean±s.e.m. C and F show arbitrary units.
    Mouse Antibodies Against Centrin2 04 1624, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+antibodies+against+centrin2+04-1624/mouse+monoclonal+antibodies+against+centrin/pmc12148038-162-27-32
    Average 90 stars, based on 1 article reviews
    mouse antibodies against centrin2 04-1624 - by Bioz Stars, 2026-09
    90/100 stars

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    1) Product Images from "Enhancement of CEP215 dynamics for spindle pole assembly during mitosis"

    Article Title: Enhancement of CEP215 dynamics for spindle pole assembly during mitosis

    Journal: Journal of Cell Science

    doi: 10.1242/jcs.263542

    Generation of CEP215-truncated mutants with defects in molecular dynamicity. (A) Schematic diagram illustrating the CEP215 protein with functional sites (CM1 and CM2), protein-binding sites, coiled-coil domains (CCDs), intrinsically disordered regions (IDRs) and the truncated mutant sites used in this study. (B) The number of cells with (w/) cytoplasmic clusters of FLAG–CEP215–CRY2-truncated mutant proteins was counted. * P <0.05 (one-way ANOVA with Tukey's post hoc test). (C) FRAP signals of the mCh–CEP215–CRY2 mutant proteins at the cytoplasm were measured for up to 600 s. w/o, without. (D) Stably expressed FLAG–mCh–CEP215 mutants in p53 KO ; CEP215 KO HeLa cells were subjected to immunoblot analyses with anti-CEP215, FLAG and GAPDH antibodies. Blot representative of four experimental repeats. (E) FRAP analyses of the FLAG–mCh–CEP215 proteins were carried out at mitotic phase centrosomes of the rescued cells. GFP–centrin2 marks the centrosomes. The analyzed cells were photographed with differential interference contrast microscopy. Scale bars: 1 μm (FRAP); 10 μm (DIC). (F) The FRAP signals of FLAG–mCh–CEP215 (WT, ΔCCD5 and L1867S) at mitotic phase centrosomes were measured for up to 160 s. For B, C and F, at least 90 (B), 30 (C) and 10 (F) cells per experimental group were counted in three independent experiments. Values are mean±s.e.m. C and F show arbitrary units.
    Figure Legend Snippet: Generation of CEP215-truncated mutants with defects in molecular dynamicity. (A) Schematic diagram illustrating the CEP215 protein with functional sites (CM1 and CM2), protein-binding sites, coiled-coil domains (CCDs), intrinsically disordered regions (IDRs) and the truncated mutant sites used in this study. (B) The number of cells with (w/) cytoplasmic clusters of FLAG–CEP215–CRY2-truncated mutant proteins was counted. * P <0.05 (one-way ANOVA with Tukey's post hoc test). (C) FRAP signals of the mCh–CEP215–CRY2 mutant proteins at the cytoplasm were measured for up to 600 s. w/o, without. (D) Stably expressed FLAG–mCh–CEP215 mutants in p53 KO ; CEP215 KO HeLa cells were subjected to immunoblot analyses with anti-CEP215, FLAG and GAPDH antibodies. Blot representative of four experimental repeats. (E) FRAP analyses of the FLAG–mCh–CEP215 proteins were carried out at mitotic phase centrosomes of the rescued cells. GFP–centrin2 marks the centrosomes. The analyzed cells were photographed with differential interference contrast microscopy. Scale bars: 1 μm (FRAP); 10 μm (DIC). (F) The FRAP signals of FLAG–mCh–CEP215 (WT, ΔCCD5 and L1867S) at mitotic phase centrosomes were measured for up to 160 s. For B, C and F, at least 90 (B), 30 (C) and 10 (F) cells per experimental group were counted in three independent experiments. Values are mean±s.e.m. C and F show arbitrary units.

    Techniques Used: Functional Assay, Protein Binding, Mutagenesis, Stable Transfection, Western Blot, Microscopy

    Related Articles

    Immunostaining:

    Article Title: Enhancement of CEP215 dynamics for spindle pole assembly during mitosis
    Article Snippet: .. For immunostaining analyses, we used rabbit antibodies against CEP215/CDK5RAP2 (06-1398; Merck Millipore; 1:100), PCNT , CEP192 (A302-324A; Bethyl Laboratory; 1:100), α-tubulin (ab18251; Abcam), γ-tubulin (ab11317; Abcam; 1:100); mouse antibodies against centrin2 (04-1624; Merck Millipore), α-tubulin (T6199; Sigma; 1:100), FLAG (F3165; Sigma; 1:100); and goat anti-FLAG (ab1257; Abcam; 1:100) antibodies. .. Alexa Fluor 488- and 594-conjugated secondary antibodies (Invitrogen; 1:100) were also used.



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    mouse antibodies against centrin2 04-1624  (Merck KGaA)
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    Merck KGaA mouse antibodies against centrin2 04-1624
    Generation of CEP215-truncated mutants with defects in molecular dynamicity. (A) Schematic diagram illustrating the CEP215 protein with functional sites (CM1 and CM2), protein-binding sites, coiled-coil domains (CCDs), intrinsically disordered regions (IDRs) and the truncated mutant sites used in this study. (B) The number of cells with (w/) cytoplasmic clusters of FLAG–CEP215–CRY2-truncated mutant proteins was counted. * P <0.05 (one-way ANOVA with Tukey's post hoc test). (C) FRAP signals of the mCh–CEP215–CRY2 mutant proteins at the cytoplasm were measured for up to 600 s. w/o, without. (D) Stably expressed FLAG–mCh–CEP215 mutants in p53 KO ; CEP215 KO HeLa cells were subjected to immunoblot analyses with anti-CEP215, FLAG and GAPDH antibodies. Blot representative of four experimental repeats. (E) FRAP analyses of the FLAG–mCh–CEP215 proteins were carried out at mitotic phase centrosomes of the rescued cells. <t>GFP–centrin2</t> marks the centrosomes. The analyzed cells were photographed with differential interference contrast microscopy. Scale bars: 1 μm (FRAP); 10 μm (DIC). (F) The FRAP signals of FLAG–mCh–CEP215 (WT, ΔCCD5 and L1867S) at mitotic phase centrosomes were measured for up to 160 s. For B, C and F, at least 90 (B), 30 (C) and 10 (F) cells per experimental group were counted in three independent experiments. Values are mean±s.e.m. C and F show arbitrary units.
    Mouse Antibodies Against Centrin2 04 1624, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+antibodies+against+centrin2+04-1624/mouse+monoclonal+antibodies+against+centrin/pmc12148038-162-27-32
    Average 90 stars, based on 1 article reviews
    mouse antibodies against centrin2 04-1624 - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

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    Generation of CEP215-truncated mutants with defects in molecular dynamicity. (A) Schematic diagram illustrating the CEP215 protein with functional sites (CM1 and CM2), protein-binding sites, coiled-coil domains (CCDs), intrinsically disordered regions (IDRs) and the truncated mutant sites used in this study. (B) The number of cells with (w/) cytoplasmic clusters of FLAG–CEP215–CRY2-truncated mutant proteins was counted. * P <0.05 (one-way ANOVA with Tukey's post hoc test). (C) FRAP signals of the mCh–CEP215–CRY2 mutant proteins at the cytoplasm were measured for up to 600 s. w/o, without. (D) Stably expressed FLAG–mCh–CEP215 mutants in p53 KO ; CEP215 KO HeLa cells were subjected to immunoblot analyses with anti-CEP215, FLAG and GAPDH antibodies. Blot representative of four experimental repeats. (E) FRAP analyses of the FLAG–mCh–CEP215 proteins were carried out at mitotic phase centrosomes of the rescued cells. GFP–centrin2 marks the centrosomes. The analyzed cells were photographed with differential interference contrast microscopy. Scale bars: 1 μm (FRAP); 10 μm (DIC). (F) The FRAP signals of FLAG–mCh–CEP215 (WT, ΔCCD5 and L1867S) at mitotic phase centrosomes were measured for up to 160 s. For B, C and F, at least 90 (B), 30 (C) and 10 (F) cells per experimental group were counted in three independent experiments. Values are mean±s.e.m. C and F show arbitrary units.

    Journal: Journal of Cell Science

    Article Title: Enhancement of CEP215 dynamics for spindle pole assembly during mitosis

    doi: 10.1242/jcs.263542

    Figure Lengend Snippet: Generation of CEP215-truncated mutants with defects in molecular dynamicity. (A) Schematic diagram illustrating the CEP215 protein with functional sites (CM1 and CM2), protein-binding sites, coiled-coil domains (CCDs), intrinsically disordered regions (IDRs) and the truncated mutant sites used in this study. (B) The number of cells with (w/) cytoplasmic clusters of FLAG–CEP215–CRY2-truncated mutant proteins was counted. * P <0.05 (one-way ANOVA with Tukey's post hoc test). (C) FRAP signals of the mCh–CEP215–CRY2 mutant proteins at the cytoplasm were measured for up to 600 s. w/o, without. (D) Stably expressed FLAG–mCh–CEP215 mutants in p53 KO ; CEP215 KO HeLa cells were subjected to immunoblot analyses with anti-CEP215, FLAG and GAPDH antibodies. Blot representative of four experimental repeats. (E) FRAP analyses of the FLAG–mCh–CEP215 proteins were carried out at mitotic phase centrosomes of the rescued cells. GFP–centrin2 marks the centrosomes. The analyzed cells were photographed with differential interference contrast microscopy. Scale bars: 1 μm (FRAP); 10 μm (DIC). (F) The FRAP signals of FLAG–mCh–CEP215 (WT, ΔCCD5 and L1867S) at mitotic phase centrosomes were measured for up to 160 s. For B, C and F, at least 90 (B), 30 (C) and 10 (F) cells per experimental group were counted in three independent experiments. Values are mean±s.e.m. C and F show arbitrary units.

    Article Snippet: For immunostaining analyses, we used rabbit antibodies against CEP215/CDK5RAP2 (06-1398; Merck Millipore; 1:100), PCNT , CEP192 (A302-324A; Bethyl Laboratory; 1:100), α-tubulin (ab18251; Abcam), γ-tubulin (ab11317; Abcam; 1:100); mouse antibodies against centrin2 (04-1624; Merck Millipore), α-tubulin (T6199; Sigma; 1:100), FLAG (F3165; Sigma; 1:100); and goat anti-FLAG (ab1257; Abcam; 1:100) antibodies.

    Techniques: Functional Assay, Protein Binding, Mutagenesis, Stable Transfection, Western Blot, Microscopy